Flow Cytometry

Flow cytometry is a method for measuring and analyzing the physical characteristics of individual cells or particles. It consists of running single cells in suspension in front of one or more laser beams and then collecting fluorescent signals emitted by each cell as well as the diffused light. Data collected are translated into graphs or histograms and statistics concerning cell populations and parameters under study (%, CV, Fluorescence Intensity, etc.). As well, sorting instruments may be used to physically isolate cell subsets according to specific criteria and these may be used in other types of experiments.

The principal applications of flow cytometry are:

  • immunophenotyping
  • cell cycle, apoptosis study
  • cell proliferation analysis
  • intracellular cytokines analysis
  • signal transduction pathways study (phospho-proteins)
  • gene expression measurement with fluorescent proteins (GFP, YFP, etc.)
  • intracellular Ca2+, intracellular pH measurement
  • FRET
  • ROS

 

Overview of services

  • Cell sorting: 1 to 4 ways, in tubes, plates or on slides
  • Cell sorting of human samples
  • Supervised acquisition/analysis
  • Unsupervised acquisition/analysis (for experienced or frequent users)
  • High-throughput acquisition (96-and 384-well plates)
  • Analysis station with DiVa, FlowJo, FCS Express3, ModFit LT, ForeCyt
  • Training in acquisition and analysis
  • Advice on choice of fluorochromes and protocols for many applications
  • Consulting in development of new applications of flow technology

The operation of our cell sorting equipment is restricted to properly trained facility personnel.

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