Publication — IRIC

Chromatin dynamics and DNA replication roadblocks.

A broad spectrum of spontaneous and genotoxin-induced DNA lesions impede replication fork progression. The DNA damage response that acts to promote completion of DNA replication is associated with dynamic changes in chromatin structure that include two distinct processes which operate genome-wide during S-phase. The first, often referred to as histone recycling or parental histone segregation, is characterized by the transfer of parental histones located ahead of replication forks onto nascent DNA. The second, known as de novo chromatin assembly, consists of the deposition of new histone molecules onto nascent DNA. Because these two processes occur at all replication forks, their potential to influence a multitude of DNA repair and DNA damage tolerance mechanisms is considerable. The purpose of this review is to provide a description of parental histone segregation and de novo chromatin assembly, and to illustrate how these processes influence cellular responses to DNA replication roadblocks.

Publication date
May 23, 2021
Principal Investigators
Hammond-Martel I, Verreault A, Wurtele H
PubMed reference
DNA Repair (Amst) 2021;104:103140
PubMed ID
Centre de recherche de l’Hôpital Maisonneuve-Rosemont, 5415 boulevard de l’Assomption, Montreal, H1T 2M4, Canada.