Publication — IRIC

Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking.

The nuclear pore complex is the major conduit for trafficking between the nucleus and cytoplasm. Nuclear import and export of both proteins and RNAs represent important functional steps for many biological processes. One of the major means to study NPC activity and the nuclear and cytoplasmic distribution of proteins and RNAs is through biochemical fractionation. Here, we describe detailed methods to generate high quality nuclear and cytoplasmic fractions simultaneously capturing RNA and proteins which can be used subsequently for a wide array of biochemical characterizations including proteomics and next generation sequencings.

Publication date
January 1, 2022
Principal Investigators
Culjkovic-Kraljacic B, Borden K
PubMed reference
Methods Mol Biol 2022;2502:91-104
PubMed ID
35412233
Affiliation
Department of Pathology and Cell Biology, Institute of Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada. biljana.culjkovic@umontreal.ca.