Publication — IRIC

A combined approach identifies a limited number of new thyroid hormone target genes in post-natal mouse cerebellum.

Thyroid hormones act directly on gene transcription in the post-natal developing cerebellum, controlling neuronal, and glial cell differentiation. We have combined three experimental approaches to identify the target genes that are underlying this phenomenon: 1) a microarray analysis of gene expression to identify hormone responsive genes in the cerebellum of Pax8-/- mice, a transgenic mouse model of congenital hypothyroidism; 2) a similar microarray analysis on primary culture of cerebellum neurons; and 3) a bioinformatics screen of conserved putative-binding sites in the mouse genome. This identifies surprisingly a small set of target genes, which, for some of them, might be key regulators of cerebellum development and neuronal differentiation.

Date de publication
1er juillet 2007
Chercheurs
Quignodon L, Grijota-Martinez C, Compe E, Guyot R, Allioli N, Laperrière D, Walker R, Meltzer P, Mader S, Samarut J, Flamant F
Référence PubMed
J. Mol. Endocrinol. 2007;39(1):17-28
ID PubMed
17601882
Affiliation
Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université de Lyon, UMR INRA CNRS 5242, IFR128 46 allée d’Italie, 69364 Lyon Cedex 07, France.