Publication — IRIC

Genome-wide approaches for identification of nuclear receptor target genes.

Large-scale genomics analyses have grown by leaps and bounds with the rapid advances in high throughput DNA sequencing and synthesis techniques. Nuclear receptor signaling is ideally suited to genomics studies because receptors function as ligand-regulated gene switches. This review will survey the strengths and limitations of three major classes of high throughput techniques widely used in the nuclear receptor field to characterize ligand-dependent gene regulation: expression profiling studies (microarrays, SAGE and related techniques), chromatin immunoprecipitation followed by microarray (ChIP-on-chip), and genome-wide in silico hormone response element screens. We will discuss each technique, and how each has contributed to our understanding of nuclear receptor signaling.

Date de publication
1er janvier 2006
Chercheurs
Tavera-Mendoza LE, Mader S, White JH
Référence PubMed
2006;4:e018
ID PubMed
16862224
Affiliation
Department of Medicine, McGill University, Montreal, Quebec, Canada.