The Histology platform covers the study and analysis of morphological and functional changes in tissues and provides a better understanding of cancer mechanisms. It enables the preservation of tissue samples taken from patients or animals through embedding (paraffin or OCT on frozen samples), sectioning, and staining. It also meets the needs for the preparation of cell pellets and tissue microarrays. Finally, it offers the acquisition of complete images of microscope slides.
Services offered:
- Preparation of fresh tissue, cryostat sections, routine H&E staining
- Tissue fixation, paraffin embedding, microtome sections and routine H&E staining
- Preparation of cell pellets in paraffin
- Special staining
- Immunohistochemistry and immunofluorescence
- Preparation of tissue microarrays (TMA)
- Digitization of clear field and fluorescence sections at 10, 20, and 40X
and - Microscopic analysis of human, murine, and other experimental model tissues by a pathologist
- Histology consulting
- Cryostatic cuts, to obtain a rapid diagnosis or solve fixation problems threatening the integrity of cellular constituents
- Tissue fixation, paraffin coating, microtome cutting and routine coloration – the first steps in the process of microscopic tissue examination
- Histochemistry and special colorations to better identify certain constituents of the cell and extracellular matrix (list of colorations available upon request)
- Immunohistochemistry (IHC) and in situ hybridization (ISH) (Antibody catalogue frequently updated and available upon request)
- Creation of tissue microarrays (TMA) allowing analysis of several hundred tissue samples simultaneously
- Access to MTA-1 (Beecher)
- Laser microdissection to isolate a sub-population of cells within a complex tissue environment and undertake more precise studies (e.g., search for mutations)
- Access to PALM MicroBeam (Zeiss)
- Image acquisition and analysis to better document the lesions present in histological preparations through the automated quantification of immunohistochemical markers.
- Access to Visiopharm, quantitative digital pathology analysis
- Microscopic analysis by a pathologist of tissues from human, mouse and any other experimental systems to interpret the results from histological cuts and other techniques
- Digitalization of the cuts and virtual microscopy, making it possible to store data and share information, as well as facilitate the consultation, archiving and future study of macroscopic and microscopic lesions. MAC/PC compatible.
- Access to NanoZoomer 2.0-HT (Hamamatsu)
Equipment:
- Leica CM3050SA cryostat
- Sakura Tissue Tek VIP6 circulator
- Leica RM2255 and Shandon Finesse E+ microtomes
- Sakura DRS601 stainer
- Leica Bond RX immunocolorator
- Sakura Tissue-Tek Glas slide mounters
- Beecher MTA-1 tissue chip preparation module
- Hamamatsu NanoZoomer 2.0-HT slide scanner (capacity 210 slides)
- Slide scanner + fluorescence (DAPI-AF488-AF555-AF647-AF750) Evident VS200 (capacity 60 slides)
Complete the form, save a PDF copy, print a paper copy that you’ll bring with your samples.