Pierre Thibault’s team shares its knowledge on mass spectrometry-based proteomic strategies used to study UBL like modifications
Published on July 29, 2021
The group of Pierre Thibault, Principal Investigator at IRIC and professor in the Department of Chemistry of UdeM, with a colleague from Leiden University Medical Center in the Netherlands, recently published an exhaustive review article in Nature Reviews Methods Primers to educate researchers of all levels on the variety of mass spectrometry-based proteomic strategies used to study ubiquitin-like (UBL) modifications and the type of information that can be generated through these approaches.
Within cells, the activity of many proteins is governed by different reversible chemical modifications. Ubiquitin-like (UBL) modifications that involves the transfer of small proteins like Ubiquitin (Ub) and SUMO (Small Ubiquitin-like Modifier), to specific sites on the targeted proteins are particularly important in in the context of DNA repair, the cell cycle, protein quality control, and many mother biological processes, but many aspects of their regulation remain to be addressed. A broader use of mass spectrometry-based proteomics approaches, including novel techniques developed by Pierre Thibault’s group at IRIC, will provide useful information towards elucidating their roles in complex diseases such as cancer, Alzheimer’s, Parkinson’s and more.
Proteomic strategies for characterizing ubiquitin-like modifications
Chongyang Li, Trent Nelson, Alfred Vertegaal and Pierre Thibault
Nature Reviews Methods Primers