Platform

Genomics

The Genomics Core Facility offers a wide range of services, combining guidance and access to state-of-the-art technologies in the field of genomics. These services range from sample preparation, to basic sequencing, to the generation of large-scale NGS datasets.

The core facility offers complete real time PCR service and Digital Droplet PCR (ddPCR) service, with support from the RNA extraction to the final results and works closely with the IRIC’s Bioinformatics Core Facility.

For more details on the platform, or to submit a request

Contact
Raphaëlle Lambert
Phone (office)
(514) 343-6111, ext. 53313
Email
raphaelle.lambert@umontreal.ca

Services offered:

  • Capillary sequencing
  • DNA sequencing (plasmids, PCR products, etc.)
    • Results provided in 48 hours and personalized access to sequences on a secure server
  • Real time PCR (qPCR) and Digital Droplet PCR (ddPCR)
  • Next generation sequencing
    • RNA-Seq
    • Amplicons
    • Small RNAs (miRNAs)
    • Targeted genomic regions (Exome sequencing)
    • ChIP-seq (chromatin immunoprecipitation sequencing)
    • Whole genomic DNA sequencing (complete genome)
  • Access to expert advice with respect to optimal sequencing strategy and experimental design
  • Validation and analysis of results
  • DNA and RNA analysis
  • Analysis of the quality and quantity of the RNA (full report in PDF format, RIN quality index for the RNA)

Equipment

Sanger sequencing

DNA analyzer ABI 3730

Next-generation sequencing

Illumina NextSeq500
Illumina MiSeq

qPCR

Life Technologies ViiA 7
Life Technologies QuantStudio 7 Flex
Roche LC480

RNA and DNA quality analysis

Agilent Bioanalyzer 2100

Spectrophotometer

NanoDrop 8000

Digital Droplet PCR

Biorad QX200

Sonicator

Covaris S-series

Semi-automated pipetting stations

  • Biomek FX
  • EpiMotion

Staff